| Title of Work: |
Identification of Microorganisms
Present Around Bemidji State
University Through Gene Sequencing |
| Presenters, Majors: |
Maria Lucio, Biology
Uzoma Nwokoro, Biology, Nursing |
Abstract: Historically, microorganisms have been identified through
biochemical, metabolic, and microscopic evaluations. Currently, DNA
sequencing of ribosomal genes provides a rapid method for identification
of diverse microorganisms. In order to test this method of bacterial
identification, we collected a variety of specimens around the Bemidji State
University campus and surrounding areas. The microorganisms were then
cultivated onto an agar plate. Colonies were isolated from plates, and grown
in a nutrient broth agar. Some of the culture was cultivated back onto an
agar plate and treated with different types of antibiotics to determine their
resistance to a variety of antibiotics. DNA from resistant bacteria was isolated
and used in a Polymerase Chain Reaction (PCR) to amplify the ribosomal
target gene. This DNA was purified and submitted for DNA sequencing in
order to identify the cultured bacteria. |