The 15,000 members of Alpha Phi Sigma belong to the following chapters:
| Chapter | College | Place | Date |
| Alpha | Truman State University | Kirksville, Mo. | 1930 |
| Beta | Northwest Missouri State Univ. | Maryville, Mo. | 1930 |
| Gamma | Mary Washington College | Fredericksburg, Va. | 1930 |
| Delta | State Teachers | Farmville, Va. | 1930 |
| Epsilon | Central Missouri State | Warrensburg, Mo. | 1930 |
| Zeta | Western Carolina University | Cullowhee, N.C. | 1930 |
| Eta | Sam Houston State University | Huntsville, Tex. | 1931 |
| Theta | Southeastern Oklahoma St. Univ. | Duran, Okla. | 1931 |
| Iota | Southwestern Oklahoma St. Univ. | Weatherford, Okla. | 1934 |
| Kappa | Northwestern Oklahoma St. Univ. | Alva, Okla. | 1934 |
| Lambda | Central State University | Edmond, Okla. | 1934 |
| Mu | New Mexico Highlands University | Las Vegas, N.M. | 1935 |
| Nu | University of Missouri - St. Louis | St. Louis, Mo. | 1935 |
| Rho | West Liberty State College | West Liberty, W. Va. | 1935 |
| Nautilus | Bemidji State University | Bemidji, Minn. | 1939 |
| Pi | Dakota State University | Madison, S.D. | 1939 |
| Xi | Cheyney Univ. of Pennsylvania | Cheyney, Pa. | 1956 |
| Omicron | Mayville State University | Mayville, N.D. | 1956 |
| Sigma | Bluefield State College | Bluefield, W. Va. | 1956 |
| Tau | Chadron State College | Chadron, Neb. | 1957 |
| Upsilon | Central State University | Wilberforce, Ohio | 1957 |
| Phi | Teachers College | Winston-Salem, N.C. | 1957 |
| Chi | Buena Vista College | Fort Dodge, Ia. | 1982 |
| Alpha Psi | Bluefield Collge | Bluefield, Va. | 1983 |
| Mu Sigma | Morehouse College | Atlanta, Ga. | 1993 |
